Journal: bioRxiv
Article Title: Substituent-based Modulation of Self-Assembly and Immunogenicity of Amphipathic Peptides
doi: 10.1101/2025.07.08.663637
Figure Lengend Snippet: (A) Schematic representation depicting ELISA assay for IL-2 generation upon interaction of mouse BMDCs with DOBW hybridoma in the presence of 5-mer peptide nanofibers conjugated to OVA peptide. (B) Concentration dependent IL-2 production by OVA-conjugated different peptide nanofibers upon treatment in mouse BMDCs and overlayed with DOBW hybridoma for 16h. (C) Time dependent IL-2 production by OVA-conjugated different peptide nanofibers upon treatment in mouse BMDCs. p-values < 0.05 were considered statistically significant. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Article Snippet: Briefly, 96-well high-binding ELISA plates (CORNING Costar 3361) were coated with 100 μL of the capture antibody in 1× PBS (pH 7.4) overnight at 4°C and then blocked with blocking buffer (1% BSA in 1× PBS, pH 7.4) for 1h.
Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay